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2 edition of Studies on Spiroplasma citri. found in the catalog.

Studies on Spiroplasma citri.

Rodney Townsend

Studies on Spiroplasma citri.

by Rodney Townsend

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  • 11 Currently reading

Published by University of East Anglia in Norwich .
Written in English


Edition Notes

Thesis (Ph.D.) - University ofEast Anglia, School of Biological Sciences, 1977.

ID Numbers
Open LibraryOL13845758M

Transmission of Different Isolates of Spiroplasma citri to Carrot and Citrus by Circulifer tenellus (Hemiptera: Cicadellidae) A.F.S. MELLO, 1 A. C. WAYADANDE, R. K. YOKOMI, 2 . Prophages in “Candidatus Liberibacter asiaticus” and Spiroplasma citri. In: Book of Abstracts, 19th Conference of International Organization of Citrus Virologists. p Interpretive Summary: Technical Abstract: A prophage is bacteriophage DNA integrated into a bacterial chromosome or existing as a plasmid inside the bacterial cell. It.

Citrus is the main economic host of Spiroplasma name "stubborn" should be restricted to the disease in citrus. Genome characteristics: G+C content of S. citri GIIX chromosome is %. It encodes one single 16SS-5S rRNA operon (Carle et al., ).Several pathways have been identified in S. citri, including phosphoenolpyruvate Phosphotransferase System . Plant Disease / February Polymerase Chain Reaction-Based Detection of Spiroplasma citri Associated with Citrus Stubborn Disease Raymond K. Yokomi, United States Department of Agriculture–Agricultural Research Service (USDA-ARS), Par- lier, CA ; Alexandre F. S. Mello, Department of Entomology and Plant Pathology, Oklahoma State University,Cited by:

Madagascar periwinkle is an ornamental and a medicinal plant, and is also an indicator plant that is highly susceptible to phytoplasma and spiroplasma infections from different crops. Periwinkle lethal yellows, caused by Spiroplasma citri, is one of the most devastating diseases of periwinkle. The response of plants to S. citri infection is very little known at the transcriptome by: Confocal microscopy analyses of Spiroplasma citri-infected C. haematoceps salivary glands and cultured cell line (Ciha-1) experimentally infected by S. citri provided evidence that spiroplasmas associate with cell actin microfilaments. Adhesin-like proteins namely SARP1, ScARPs and SkARP are encoded on spiroplasma plasmids pBJS-O, pSci and.


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Studies on Spiroplasma citri by Rodney Townsend Download PDF EPUB FB2

Spiroplasma citri is a bacterium species and the causative agent of Citrus stubborn disease. Its genome has been partially sequenced. The restriction enzyme SciNI, with the cutting site 5' GCGC / 3' CGCG, can be found in S.

citri. Euscelis plebejus can be used as a vector of the bacterium to experimentally infect white clover (Trifolium repens). Family: Spiroplasmataceae. The results of extensive studies of the ecology of this spiroplasma suggest that it is host specific for Leptinotarsa beetles.

Strain LD-1 (= ATCC ) is designated the type strain of a new. Saglio P, et al. Spiroplasma citri gen.

and sp. n.: a mycoplasma-like organism associated with "stubborn" disease of Citrus. Int. Syst. Bacteriol.Simoneau P, Labarere J.

Isolation of Spiroplasma citri membranes and characterization of membrane proteins by two-dimensional gel electrophoresis. Curr. Spiroplama citri, the causal agent of citrus stubborn disease, is a bacterium of the class Mollicutes and is transmitted by phloem-feeding leafhopper vectors.

In order to characterize candidate genes potentially involved in spiroplasma transmission and pathogenicity, the genome of S. citri strain GIIX is currently being deciphered. Assembl sequencing reads Cited by: Jiyeun K.

Kim, Bok L. Lee, in Advances in Insect Physiology, Fruit Fly–Facultative Symbionts. Wolbachia and Spiroplasma are two known vertically transmitted facultative symbionts in Drosophila (Mateos et al., ).Not only their effects on the reproductive manipulation, Wolbachia and Spiroplasma play defensive roles against broad enemies, such as viruses.

• Spiroplasma citri S. citri forms wall-less pleomorphic cells with a characteristic spiral morphology. The minimum viable length of a helix is x µm. The helices are motile Studies on Spiroplasma citri. book flexing or rotation. Some strains are non-motile and non-helical. citri is one of the very few plant pathogenic mollicutes to have been Size: 61KB.

Kevin J. Hackett, Dwight E. Lynn, in Molecular and Diagnostic Procedures in Mycoplasmology, Coculture of Spiroplasmas with Insect Cells. Spiroplasmas can be isolated as described in Hackett and Clark (), with the technique being dependent on the location (gut or hemolymph) of the spiroplasma in the host.

Although some researchers prefer to provide as high an. Acquisition of Spiroplasma citri through membranes by Homopterous insects. Phytopathology, 65(10) Saglio P, L'Hospital M, LaflFche D, Dupont G, Bové JM, Tully JG, Freundt EA, Spiroplasma citri gen.

and sp. n.: a mycoplasma-like organism associated with stubborn disease of citrus. Abstract. Spiroplasma spp., tiny filterable wall-less bacteria, are consistently associated with the transmissible spongiform encephalopathies (TSE).

Spiral forms have been transiently isolated from TSE-affected brain tissues in SP4 growth media designed for isolation of Spiroplasma spp., but the isolate could not be propagated in SP4 media.

A bacterium must Cited by: 1. Lobsided fruit from a sweet orange tree infected by S. citri. Thickening of the albedo at the peduncular end can be observed while the albedo at the stylar end is very thin.

Courtesy: J.M. Bové, INRA Bordeaux (FR). Abstract Spiroplasma citri, the type species of the genus Spiroplasma (Spiroplasmataceae, Mollicutes), is restricted to the phloem sieve tubes and transmitted by phloem sap-feeding insects, as is characteristic of the phytopathogenic mollicutes. The spiroplasmas are the only mollicutes showing motility and helical morphology, apparently Cited by: Spiroplasma citri is a phloem-limited bacterium causing citrus stubborn disease (CSD).

Isolation and culturing of S. citri is technically demanding and time consuming.S. citri is typically low in titer and unevenly distributed in citrus, making reliable detection challenging.

The current preferred detection method is polymerase chain reaction (PCR) assays with primers developed from. This is the final report for project D, "Characterization, Epidemiology and Management Strategies of Citrus tristeza virus and Spiroplasma citri on Citrus in California," which has been replaced by new project DD, "Characterization and Management of Citrus Pathogens Transmitted by Phloem-Feeding Insect Vectors.".

A physical and genetic map of the Spiroplasma citri genome has been constructed using several restriction enzymes and pulsed field gel electrophoresis.

A number of genes were subsequently localized on the map by the use of appropriate probes. The genome size of the spiroplasma estimated from restriction fragments is close to kbp, the Cited by: Background.

Spiroplasma citri is a cell wall-less, plant pathogenic bacteria that colonizes two distinct hosts, the leafhopper vector and the host plant. Given the absence of a cell wall, surface proteins including lipoproteins and transmembrane polypeptides are expected to play key roles in spiroplasma/host by: 2.

Xanthomonas citri subsp. citri (ex Hasse) Gabriel et al. (syns. axonopodis pv. citri Hasse; X. campestris pv. citri (Hasse) Dye) Citrus variegated chlorosis Xylella fastidiosa Wells et al. Huanglongbing (Greening) ‘Candidatus Liberibacter africanus' ‘Ca. americanus' ‘Ca. asiaticus' Stubborn Spiroplasma citri Saglio.

Spiroplasma citri causes stubborn disease in Citrus spp. and diseases in other plants. Here, we report the nucleotide sequence of the 1,bp circular chromosome and two plasmids of S.

citri strain R8-A2(T) This information will facilitate analyses to understand spiroplasmal pathogenicity and evolutionary adaptations to lifestyles in plants and arthropod. Spiroplasma citri ATCC ® D-5™ Designation: Genomic DNA from Spiroplasma citri strain Morocco-R8-A2, NCPPB TypeStrain=True Application.

studies on plant response to phytoplasma infection Journal of Plant Pathology (), 88 (2), Edizioni ETS Pisa, INVITED REVIEW SUGAR METABOLISM AND PATHOGENICITY OF SPIROPLASMA CITRI J. Renaudin UMR Génomique Développement et Pouvoir Pathogène, INRA-Université de Bordeaux2, I.B.V.M.

Spiroplasma citri is a phloem-limited bacterium that causes citrus stubborn disease and vegetative disorders in Apiaceae, such as carrot and celery, and in other hosts.

Detection methods include indexing in indicator plants, in vitro culturing in specific media, and serological and molecular amplification methods, e.g., conventional and real Cited by: 1. The deformation test is a simple and highly sensitive technique capable of demonstrating significant antigenic differences among helical, wall-less prokaryotes (spiroplasmas).

Specific identified. Quantitative relationships among various antisera are determined by examining, under dark-field microscopy, samples containing serum dilutions Cited by: Species-specific and cross-reactive monoclonal antibodies to the plant-pathogenic spiroplasmas Spiroplasma citri and S.

kunkelii. Phytopathology, 79(8) Lee IM; Davis RE, New developments in the culture of Spiroplasma kunkelii, the corn stunt spiroplasma. Mycoplasma diseases of crops.Studies of symptomatology and detection of Spiroplasma citri, a causal agent of stubborn disease, observed on citrus trees in the East Mediterranean area.

Foreign Title: Doğu akdeniz bölgesi'nde turunçgil ağaçlarında gözlenen stubborn hastalığı etmeni Spiroplasma citri'nin simptomoloji ve tanılanması üzerinde : K. Çağlayan, A. Çınar.